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Gas chromatography–mass spectrometry based metabolic profiling for the identification of discrimination markers of Angelicae Radix and its application to gas chromatography–flame ionization detector system

Paper ID Volume ID Publish Year Pages File Format Full-Text
21174 43209 2012 5 PDF Available
Title
Gas chromatography–mass spectrometry based metabolic profiling for the identification of discrimination markers of Angelicae Radix and its application to gas chromatography–flame ionization detector system
Abstract

Gas chromatography (GC)-based metabolomics technologies were applied for quality control of Angelicae Radix, an herbal medicine commonly used in Japan and China. Since Angelica roots are priced and graded differently based on their species and cultivation area, there is a need for a simple and reproducible method to discriminate Angelica roots. Here, we used GC–MS profiling data to construct a discrimination method for species and cultivation area of A. Radix. Seventy-six primary metabolites were identified. The quality factors of A. Radix were successfully classified using metabolic profiling and the orthogonal projections to latent structures-discriminant analysis (OPLS-DA) technique. Sorbitol and a glucose/4-aminobutyric acid combination were chosen as bio-markers from S-plot of OPLS-DA. Application of these selected bio-markers to a more practical and cost-efficient system, namely gas chromatography–flame ionization detector (GC–FID) system were also assessed. As a result, the same separations of sorbitol, glucose and 4-aminobutyric acid in box plots were obtained from GC–FID data. Our results demonstrate that GC-based metabolic markers can be readily applied for the establishment of a practical quality control method for A. Radix.

Keywords
Angelicae Radix; Gas chromatography–mass spectrometry (GC–MS); Gas chromatography–flame ionization detector (GC–FID); Metabolomics; Quality control
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Gas chromatography–mass spectrometry based metabolic profiling for the identification of discrimination markers of Angelicae Radix and its application to gas chromatography–flame ionization detector system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 114, Issue 2, August 2012, Pages 232–236
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us