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Construction of a Saccharomyces cerevisiae strain with a high level of RNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
21177 43210 2011 7 PDF Available
Title
Construction of a Saccharomyces cerevisiae strain with a high level of RNA
Abstract

A strategy has been developed for creating Saccharomyces cerevisiae strains with a high RNA content by following a three-step breeding procedure. In the first step, an S. cerevisiae disruptant of the RRN10 gene, one of the components of the UAF (upstream activation factor) complex of rRNA transcription, was constructed and showed severely slow growth. In the second step, seven suppressors were isolated that restored the slow growth of the Δrrn10 disruptant. Genetic analysis revealed that each of the seven suppressors that were isolated appeared to have dominant and multiple mutations. The specific growth rate of those suppressors was increased approximately two-fold as compared with the Δrrn10 parental strain. The absolute RNA content showed that the suppressors had an RNA content 32–56% higher than that of the Δrrn10 parental strain. In the last step, the RRN10 wild-type gene was integrated into chromosome V of each of the original suppressors. The total RNA content of the integrants was also 1.4- to 2.3-fold higher than the wild-type strain. In conclusion, since yeast RNA is the source of 5′-IMP and 5′-GMP that enhance the delicious taste in certain types of food, like soups and sauces, the strategy taken in this study provides effective approach to breed S. cerevisiae strains producing a higher content of RNA that will contribute to yeast food biotechnology.

Keywords
Saccharomyces cerevisiae; Ribosomal RNA; RRN10 gene; Suppressor; Strain improvement
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Construction of a Saccharomyces cerevisiae strain with a high level of RNA
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 1, July 2011, Pages 1–7
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us