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Acetate–glycerol cometabolism: Cultivating Schizosaccharomyces pombe on a non-fermentable carbon source in a defined minimal medium

Paper ID Volume ID Publish Year Pages File Format Full-Text
21180 43210 2011 6 PDF Available
Title
Acetate–glycerol cometabolism: Cultivating Schizosaccharomyces pombe on a non-fermentable carbon source in a defined minimal medium
Abstract

ABSTRACTThe growth of the fission yeast Schizosaccharomyces pombe on glucose and glycerol was monitored on-line in shake flasks and microtiter plates. The Edinburgh Minimal Medium 2 was improved by doubling its concentrations, improving its buffer and increasing its sulphur and iron concentrations additionally. By growing S. pombe on mixed carbon sources, it was shown that glycerol and glucose complement one another. Several tests were performed to establish the cultivation of S. pombe with non-fermentable glycerol as the main carbon source in minimal medium. Interestingly, a synergistic effect of glycerol and acetate was discovered which can significantly improve the growth of the fission yeast on glycerol. S. pombe showed optimal respiration activity, growth, and product formation by co-utilizing 20 g/L glycerol and 2.5 g/L sodium acetate.

Keywords
Schizosaccharomyces pombe; Fission yeast; Cometabolism; Glycerol; Acetate; Minimal medium; Respiration activity; Media optimization
First Page Preview
Acetate–glycerol cometabolism: Cultivating Schizosaccharomyces pombe on a non-fermentable carbon source in a defined minimal medium
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 1, July 2011, Pages 20–25
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering