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Synergistic inhibition effect of 2-phenylethanol and ethanol on bioproduction of natural 2-phenylethanol by Saccharomyces cerevisiae and process enhancement

Paper ID Volume ID Publish Year Pages File Format Full-Text
21181 43210 2011 6 PDF Available
Title
Synergistic inhibition effect of 2-phenylethanol and ethanol on bioproduction of natural 2-phenylethanol by Saccharomyces cerevisiae and process enhancement
Abstract

Natural 2-phenylethanol (PEA) could be produced on a large scale by way of bioconversion with yeast from l-phenylalanine. In this work the synergistic inhibition effect of the target product PEA and the byproduct ethanol on the bioconversion rate by Saccharomyces cerevisiae R-UV3 was systematically studied and a new kinetic model with an item representing the synergistic effect was proposed. Optimization strategies to repress the inhibition effect of PEA and ethanol were carried out in the mode of fed-batch culture with ISPR. The glucose concentration was regulated at the level of 0.2 ± 0.1 g/L by controlling a suitable respiratory quotient on line, which could limit the accumulation of the ethanol lower than 10 g/L. In the presence of resin FD0816 with a weight of 10% of the medium, PEA was removed from the broth and the overall PEA concentration and the space-time yield reached 13.7 g/L and 0.39 g L− 1 h− 1 respectively. The semi-continuous process with ISPR was performed, in which the replacement of the resin was operated repeatedly when the aqueous PEA was over 2.7 g/L and bioconversion continued until the bioactivity of the yeast cells declined, consequently achieving a final overall PEA concentration of 32.5 g/L and a space-time yield of 0.45 g L− 1 h− 1.

Keywords
Natural 2-phenylethanol; Bioconversion; Synergistic inhibition; In situ product removal; Resin; Saccharomyces cerevisiae
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Synergistic inhibition effect of 2-phenylethanol and ethanol on bioproduction of natural 2-phenylethanol by Saccharomyces cerevisiae and process enhancement
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 1, July 2011, Pages 26–31
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us