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Fungicidal efficiency of electrolyzed oxidizing water on Candida albicans and its biochemical mechanism

Paper ID Volume ID Publish Year Pages File Format Full-Text
21193 43210 2011 6 PDF Available
Title
Fungicidal efficiency of electrolyzed oxidizing water on Candida albicans and its biochemical mechanism
Abstract

ABSTRACTThe fungicidal influencing factors of electrolyzed oxidizing water (EOW) on Candida albicans were investigated by suspension quantitative germicidal tests. Results showed that EOW possessed predominant fungicidal rate on C. albican, as high as consummately 100% after 0.5 min duration of 65.5 mg/L active available chlorine concentration (ACC). The fungicidal effect was promoted proportionally along with ACC but was inhibited by organic interferential bovine serum albumin (BSA). The fungicidal mechanism was also investigated at a biological molecular level by detecting series of biochemical indices. Fluorescent microscopy showed that almost all C. albicans cells were stained red in 1 min, suggesting that cell membrane was one of EOW's action targets. Transmission electron microscopy (TEM) showed that EOW destroyed the cellular protective barriers and imposed some damage upon the nucleus area, which verified EOW's effects on microbial ultra-structures. EOW improved membrane permeabilities with the result that the leakages of cellular inclusions (K+, proteins and DNA) and the conductivity increased rapidly. The dehydrogenase relative activities of C. albicans decreased by 44.0% after 10 min, indicating that EOW also had a destructive effect on cellular dehydrogenase.

Keywords
Electrolyzed oxidizing water; Candida albicans; Fungicidal effect; Biochemical mechanism
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Fungicidal efficiency of electrolyzed oxidizing water on Candida albicans and its biochemical mechanism
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 1, July 2011, Pages 86–91
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us