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Improvement of the transcriptional strength of baculovirus very late polyhedrin promoter by repeating its untranslated leader sequences and coexpression with the primary transactivator

Paper ID Volume ID Publish Year Pages File Format Full-Text
21200 43211 2012 3 PDF Available
Title
Improvement of the transcriptional strength of baculovirus very late polyhedrin promoter by repeating its untranslated leader sequences and coexpression with the primary transactivator
Abstract

Modified polyhedrin promoter (Ppolh) was designed by repeating burst sequences (BSs) and adopted to overexpress rat α2,6-sialyltransferase (ST6Gal I) in silkworm. Modified Ppolh of five BSs with VLF-1 coexpression yielded 2.9 U/ml ST6Gal I activity and 32.5 mU/mg specific activity, which was 1.7- and 2.3-fold higher, respectively compared to Ppolh.

Keywords
Burst sequence; Very late transcriptional factor-1; Polyhedrin promoter; Baculovirus; Silkworm
First Page Preview
Improvement of the transcriptional strength of baculovirus very late polyhedrin promoter by repeating its untranslated leader sequences and coexpression with the primary transactivator
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 6, June 2012, Pages 694–696
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering