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Biochemical profile of halophilous microalgae strains from high-andean extreme ecosystems (NE-Chile) using methodological validation approaches

Paper ID Volume ID Publish Year Pages File Format Full-Text
21207 43211 2012 7 PDF Available
Title
Biochemical profile of halophilous microalgae strains from high-andean extreme ecosystems (NE-Chile) using methodological validation approaches
Abstract

The main goal of this work is to characterize the biochemical profile of three halophilous microalgal strains isolated from extreme ecosystems in northern Chile to assess their potential as possible sources of technological and commercial advantages. A procedure for the validation of a routine method for determining the total protein, carbohydrate, lipid and chlorophyll a (Chl-a) contents in Chlorella fusca, Dunaliella salina and Spirulina sp. has been developed. Detection limits of 10.8 μg/mg, 2.7 μg/mg and 5.6 μg/mg for total protein content and 3.5 μg/mg, 1.8 μg/mg and 8.8 μg/mg for total carbohydrate content were determined in C. fusca, D. salina and Spirulina sp., respectively. Most of the biological macromolecule levels measured in the microalgae were in accordance with the magnitude of previously reported data for other strains of the same taxa. However, lower levels than expected of lipids and Chl-a were measured in C. fusca and Spirulina sp., which may be associated with an imbalance between specific growth rates and the rate of macromolecule synthesis. The protein values measured in Spirulina sp. (52.3 ± 2.2 μg/mg DW) were close to the lower limit of the range reported in the literature for non-halophilous strains of the same genus. Except for the Chl-a quantification procedure, the analytical methods for macromolecules had a high degree of repeatability and reproducibility. The variability among repeated measurements of Chl-a was associated with auto-degradation processes during pigment extraction.

Keywords
Chlorella; Dunaliella; Spirulina; Biochemical profile; Hypersaline ecosystem; Methods validation
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Biochemical profile of halophilous microalgae strains from high-andean extreme ecosystems (NE-Chile) using methodological validation approaches
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 6, June 2012, Pages 730–736
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us