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Characterisation of fermentation of high-gravity maize mashes with the application of pullulanase, proteolytic enzymes and enzymes degrading non-starch polysaccharides

Paper ID Volume ID Publish Year Pages File Format Full-Text
21224 43212 2010 6 PDF Available
Title
Characterisation of fermentation of high-gravity maize mashes with the application of pullulanase, proteolytic enzymes and enzymes degrading non-starch polysaccharides
Abstract

The aim of the research was to assess the possibility of the fermentation productivity rising through the increase in corn mashes extract from 16–17 to 20–21°Balling, yet keeping a 3-day fermentation period. The second goal was to obtain the highest possible utilization of starch in the raw material through deep enzymatic degradation and utilization of available sugars and simultaneous maintenance of high quality spirit. It was found that fulfilling the above during the 3-day fermentation period was possible with the application of pullulanase as an additional amylolytic enzyme. Adding pullulanase resulted in the acceleration of the starch hydrolysis degree, which led to lower amounts of unhydrolyzed dextrins and higher ethanol yield. When the supportive enzymes complex (pullulanase, protease and cellulase) was used, the final ethanol concentration reached 10.86 ± 0.04% v/v, with ethanol yield at 68.41 ± 0.23 dm3 of absolute ethanol (A100) per 100 kg of starch, which was 95.25 ± 0.32% at the theoretical value. The acceleration of starch enzymatic degradation and the application of a proteolytic preparation visibly shortened both initial and main fermentation phases. This in turn increased the time of the final fermentation phase and resulted in more extensive utilization of substrates by yeasts with simultaneous reduction of the final concentration of acetaldehyde (26.0 ± 0.5 mg/dm3A100) and diethyl acetal of acetaldehyde (2.5 ± 0.5 mg/dm3A100). The quality of spirit obtained was positively verified also in terms of relatively low concentration of higher alcohol (3912.2 ± 9.8 mg/dm3A100). Preliminary analysis of costs (without raw-material) of 1 l distillate production indicated the possibility to reduce the costs by 18–20%.

Keywords
Ethanol; Alcoholic fermentation; High-gravity mashes; Pullulanase; Alcoholic fermentation by-products
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Characterisation of fermentation of high-gravity maize mashes with the application of pullulanase, proteolytic enzymes and enzymes degrading non-starch polysaccharides
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 5, May 2010, Pages 466–471
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us