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Analysis of a change in bacterial community in different environments with addition of chitin or chitosan

Paper ID Volume ID Publish Year Pages File Format Full-Text
21225 43212 2010 7 PDF Available
Title
Analysis of a change in bacterial community in different environments with addition of chitin or chitosan
Abstract

The temporal changes of a bacterial community in soil with chitin or chitosan added were analyzed by PCR-denaturing gradient gel electrophoresis (DGGE) targeting the 16S rRNA gene using total DNAs prepared from the community. Band patterns of PCR-DGGE confirmed that 31 species become predominant after the addition of chitin or chitosan. The determination of the nucleotide sequences of the bands of the 31 species indicated that 20 species belonged to the division Proteobacteria, and that the genus Cellvibrio was apparently predominant among them (7/20). The 16S rRNA sequences of the 16 deduced species (16/31) showed less than 98% similarities to those of previously identified bacteria, indicating that the species were derived from unidentified bacteria. The total community DNAs extracted from bacterial cells adsorbed on the surface of flakes of chitin and chitosan placed in a river, a moat, or soil were subjected to PCR-DGGE to examine the extent of diversity of chitinolytic bacteria among different environments. The predominant species significantly differed between the chitin and chitosan placed in the river and moat, but not so much between those placed in the soil. The large difference between the diversities of the three bacterial communities indicated that a wide variety of bacteria including unidentified ones are involved in the degradation of chitin and chitosan in the above-mentioned natural environments.

Keywords
Bacterial community; PCR-DGGE; 16S rRNA gene; Chitin; Chitosan; Unculturable bacteria
First Page Preview
Analysis of a change in bacterial community in different environments with addition of chitin or chitosan
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 5, May 2010, Pages 472–478
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering