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Noninvasive discrimination of human normal cells and malignant tumor cells by phase-shifting laser microscopy

Paper ID Volume ID Publish Year Pages File Format Full-Text
21229 43212 2010 5 PDF Available
Title
Noninvasive discrimination of human normal cells and malignant tumor cells by phase-shifting laser microscopy
Abstract

The noninvasive discrimination of cancer cells from normal cells in adherent culture by the measuring of the phase shift using phase-shifting laser microscopy (PLM) was investigated with the aim of noninvasive quality control of cell processing for transplantation. A human prostatic carcinoma epithelial cell line (PC-3) and human hepatocellular carcinoma cell lines (Hep3B, PLC, HLF, and Huh7) showed markedly lower phase shifts as measured by PLM than those of human prostate epithelial cells (PREC) and cryopreserved human hepatocytes, respectively, although there was no apparent difference in cell morphology between these sets of cells. Mixed cultures of PC-3 and PREC, in which the percentages of PC-3 cells were determined to be 43.3%, 14.0%, and 10.6% by immunofluorescence staining, were prepared, and the percentages were calculated to be 50.0%, 8.0%, and 2.5% using the distribution of the phase shift data of the cells, respectively. In conclusion, these adherent cancer cells can be noninvasively discriminated from normal adherent cells by phase shift measurement using PLM, and the sensitivity of the method of detecting cancer cell contamination reached 10%.

Keywords
Noninvasive; Discrimination; Malignant tumor cells; Phase shifting; Regenerative medicine; Cancer cells; Quality control; Microscope
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Noninvasive discrimination of human normal cells and malignant tumor cells by phase-shifting laser microscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 5, May 2010, Pages 499–503
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us