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Cln3 blocks IME1 transcription and the Ime1–Ume6 interaction to cause the sporulation incompetence in a sake yeast, Kyokai no. 7

Paper ID Volume ID Publish Year Pages File Format Full-Text
21232 43213 2010 7 PDF Available
Title
Cln3 blocks IME1 transcription and the Ime1–Ume6 interaction to cause the sporulation incompetence in a sake yeast, Kyokai no. 7
Abstract

SummaryIndustrial yeasts, including a sake yeast Kyokai no. 7 (K7), are generally unable to sporulate. In K7 (Saccharomyces cerevisiae) cells, IME1 transcription was not induced under sporulation conditions, and K7 cells partially restored sporulation ability when transformed with a multicopy plasmid bearing IME1. However, the mechanisms of sporulation incompetence in industrial yeasts are poorly understood. We demonstrated that the deletion of the G1 cyclin CLN3, a key activator of the cell cycle, allows K7 cells to induce IME1 transcription and sporulate under sporulation conditions. In K7 cells, CLN3 mRNA and protein were not down-regulated despite sporulation conditions. Moreover, using a two-hybrid assay, we found that Ime1–Ume6 interaction was promoted in Cln3-deficient K7 cells. Thus, Cln3 is involved in the mechanism underlying sporulation incompetence by inhibiting IME1 transcription and the Ime1–Ume6 interaction. Based on these findings, we hypothesize that the absence of transmission of nutrient starvation signals to CLN3 leads to sporulation incompetence in K7 cells.

Keywords
Cln3 Sporulation incompetence Kyokai no. 7
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Cln3 blocks IME1 transcription and the Ime1–Ume6 interaction to cause the sporulation incompetence in a sake yeast, Kyokai no. 7
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 1, July 2010, Pages 1–7
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us