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In vitro behaviour of adult mesenchymal stem cells seeded on a bioactive glass ceramic in the SiO2–CaO–P2O5 system

Paper ID Volume ID Publish Year Pages File Format Full-Text
2124 100 2008 10 PDF Available
Title
In vitro behaviour of adult mesenchymal stem cells seeded on a bioactive glass ceramic in the SiO2–CaO–P2O5 system
Abstract

This work describes the evaluation of a glass ceramic (55S41C4P-1300) as a potential substrate for bone tissue engineering. For that purpose, the capacity of mesenchymal stem cells (MSCs), isolated from rabbit bone marrow, to adhere, proliferate and differentiate into osteoblast (OBs) with or without 55S41C4P-1300 was investigated. Two types of culture medium, i.e. growth medium (GM) and osteogenic medium (OM), were evaluated. The bioactive 55S41C4P-1300, containing pseudowollastonite, wollastonite, tricalcium phosphate and crystoballite as crystalline phases, was obtained by heat treatment of a sol–gel glass (55SiO2, 41CaO, 4P2O5 (mol.%)) at 1300 °C.The results showed that the MSCs adhered, spread, proliferated and produced mineralized extracellular matrix on 55S41C4P-1300 regardless of the culture medium used. As the same time, they showed an osteoblastic phenotype, and this phenomenon was accompanied by the gradual diminution of the marker CD90 expression. The 55S41C4P-1300 was able to induce the differentiation of MSCs into OBs in the same way as OM without glass ceramic. This effect increased with the combination of 55S41C4P-1300 with OM. The glass ceramic evaluated in this work is bioactive, cytocompatible and capable of promoting the differentiation of MSCs into OBs. For that reason, it could be regarded as a suitable matrix in tissue engineering for bone tissue regeneration.

Keywords
Glass ceramic; Scaffold; Bone tissue engineering; Mesenchymal stem cell; Osteoblast
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In vitro behaviour of adult mesenchymal stem cells seeded on a bioactive glass ceramic in the SiO2–CaO–P2O5 system
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 4, Issue 4, July 2008, Pages 1104–1113
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us