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New monitoring approach for metabolic dynamics in microbial ecosystems using stable-isotope-labeling technologies

Paper ID Volume ID Publish Year Pages File Format Full-Text
21247 43213 2010 7 PDF Available
Title
New monitoring approach for metabolic dynamics in microbial ecosystems using stable-isotope-labeling technologies
Abstract

We have developed a new approach for monitoring the metabolic dynamics in microbial ecosystems using a combination of DNA fingerprinting and metabolome analysis based on stable-isotope-labeling technologies. Stable-isotope probing of DNA (DNA-SIP) has been used previously for the evaluation of cross-feeding in microbial communities. For the development and validation of our monitoring approach, fecal microbiota were analyzed with stable-isotope-labeled glucose used as the sole carbon source. In order to link the metabolic information and the microbial variability, we performed metabolic–microbial correlation analysis based on nuclear magnetic resonance (NMR) profiles and denaturing gradient gel electrophoresis (DGGE) fingerprints, which successfully identified the glucose-utilizing bacteria and their related extracellular metabolites. Moreover, our approach revealed information regarding the carbon flux, in that the “first” wave of extracellular metabolites secreted by the glucose-utilizing bacteria were incorporated into the “secondary” group of substrate-utilizing bacteria, and that this “secondary” group further produced their own secondary metabolized substrates. Thus, this approach is a powerful tool for monitoring the metabolic dynamics in microbial ecosystems and allows for the tracking of the carbon flux within a microbial community.

Keywords
Nuclear magnetic resonance (NMR); Stable-isotope probing (SIP); Metabolic dynamics; Carbon flux; Denaturing gradient gel electrophoresis (DGGE)
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New monitoring approach for metabolic dynamics in microbial ecosystems using stable-isotope-labeling technologies
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 1, July 2010, Pages 87–93
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us