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Intracellular co-expression of Vitreoscilla hemoglobin enhances cell performance and β-galactosidase production in Pichia pastoris

Paper ID Volume ID Publish Year Pages File Format Full-Text
21265 43214 2012 6 PDF Available
Title
Intracellular co-expression of Vitreoscilla hemoglobin enhances cell performance and β-galactosidase production in Pichia pastoris
Abstract

Pichia pastoris has been used to produce various recombinant proteins under high oxygen demand conditions. To improve the heterologous production of β-galactosidase, the vgb gene encoding Vitreoscilla hemoglobin (VHb) was co-expressed in the P. pastoris cytoplasm under the control of the methanol-inducible promoter. Co-expression of VHb under different aeration conditions improved cell performance in terms of growth, viability, respiratory rate, and β-galactosidase production. Under limiting aeration conditions, the VHb+ strain produced 28.2% more biomass but 31.2% less total β-galactosidase activity than the VHb− strain. Under non-limiting aeration conditions, the VHb+ strain showed 20.3% higher cell growth and 9.9% more total β-galactosidase activity than the VHb− strain. Moreover, under these conditions, the VHb+ strain was 7.7% more viable and had a 28.2% higher oxygen uptake rate (OUR) than the VHb− strain. Evidently, VHb can enhance the OUR and promote methanol metabolism, thereby improving cell performance and β-galactosidase production.

Keywords
Pichia pastoris; Vitreoscilla hemoglobin; β-galactosidase; AOX1 promoter; Cell performance; Recombinant protein expression
First Page Preview
Intracellular co-expression of Vitreoscilla hemoglobin enhances cell performance and β-galactosidase production in Pichia pastoris
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 3, March 2012, Pages 332–337
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering