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Changes in the gene expression profile of Acetobacter aceti during growth on ethanol

Paper ID Volume ID Publish Year Pages File Format Full-Text
21267 43214 2012 6 PDF Available
Title
Changes in the gene expression profile of Acetobacter aceti during growth on ethanol
Abstract

Acetobacter aceti NBRC 14818 shows a diauxic growth profile and temporarily accumulates acetate when grown in medium containing ethanol. However, the mechanisms underlying the metabolic switching between the incomplete oxidation of ethanol and overoxidation of acetate, and the control of stress resistance systems in A. aceti cells grown on ethanol are not fully understood. In this study, time-dependent transcriptome changes in cells during growth on ethanol were analyzed by DNA microarray. In A. aceti, ethanol is oxidized to acetate via acetaldehyde by sequential reactions of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). We found that the genes encoding pyrroloquinoline quinone-dependent ADH, membrane-bound ALDH, and two NAD+-ADHs were expressed constitutively in cells throughout the culture period. In contrast, the expression levels of genes encoding tricarboxylic acid (TCA) cycle enzymes were low during acetate accumulation until ethanol was consumed, but were significantly upregulated after the accumulated acetate was started to be consumed. This result suggests that changes in the carbon metabolic flow through the TCA cycle are important for the metabolic switching from acetate accumulation to the overoxidation of acetate. In addition, the genes for glyoxylate pathway enzymes were significantly upregulated soon after the cells began oxidizing ethanol, indicating that this pathway is important for the utilization of ethanol as a carbon source.

Keywords
ADH, alcohol dehydrogenase; ALDH, aldehyde dehydrogenase; PQQ, pyrroloquinoline quinone; SCACT, succinyl-CoA:acetate CoA transferaseEthanol; Acetate; Transcriptome; Oxidative fermentation; Acetobacter aceti
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Changes in the gene expression profile of Acetobacter aceti during growth on ethanol
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 3, March 2012, Pages 343–348
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us