fulltext.study @t Gmail

Effect of temperature on the stability of various peptidases during peptide-enriched soy sauce fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
21269 43214 2012 5 PDF Available
Title
Effect of temperature on the stability of various peptidases during peptide-enriched soy sauce fermentation
Abstract

We previously developed a peptide-enriched soy sauce-like seasoning called Fermented Soybean Seasoning (FSS) with high-temperature fermentation, and we have reported the antihypertensive effects of FSS. Seryl–tyrosine (Ser–Tyr) and glycyl–tyrosine (Gly–Tyr) were identified from FSS as active constituents in the antihypertensive effects. They were found to be particularly enriched in FSS; more so than in regular soy sauce. In the present study, we clarified one of the mechanisms underlying the accumulation of these bioactive peptides during high temperature soy sauce brewing. Crude enzyme extracts were prepared from model soy sauce mash (moromi) fermented at various temperatures. Leucine aminopeptidase-I, II, and seryl–tyrosine hydrolytic activity were found to decrease in the moromi incubated at the fermentation temperature of FSS whereas almost no decrease was observed in that of regular soy sauce. The concentrations of ACE inhibitory peptides, Ser–Tyr and Gly–Tyr, in the moromi incubated at high temperature were revealed to be higher than those at low temperature through quantitative LC–MS/MS analysis. These results suggested that the peptidases responsible for degrading low molecular weight bioactive peptides were inactivated during the high temperature fermentation, thus, these peptides would be likely to remain in the high temperature fermentation.

Keywords
ACE inhibitory peptide; Soy sauce; Fermentation temperature; Leucine aminopeptidase; Thermal stability
First Page Preview
Effect of temperature on the stability of various peptidases during peptide-enriched soy sauce fermentation
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 3, March 2012, Pages 355–359
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us