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Effects of mixed feeder cells on the expansion of CD34+ cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
21275 43214 2012 6 PDF Available
Title
Effects of mixed feeder cells on the expansion of CD34+ cells
Abstract

Synergistic effects of mesenchymal stem cells (MSCs) isolated from bone marrow (BM), umbilical cord blood (UCB) and periosteum, and fibroblasts as mixed feeder cells (MFCs) on the expansion of hematopoietic progenitor cells (HPCs) were investigated in serum- and exogenous cytokine-free conditions. Enriched CD34+ cells were cultured for 2 weeks over the cell lines alone, individually, or selected combinations of them. When the cells were cultured over MFCs, the maximum increase in expansion of total nucleated cells and CD34+/CD38− cells was 157.3- and 128.6-fold, respectively. Furthermore, hematopoietic cytokine such as IL-6 and chemokines (e.g., IL-8, growth related oncogene (GRO), GRO-alpha, matrix metalloproteinase (MMP)-1, and MMP-3) were significantly increased in mixed feeder cells. Based on these results, MFCs can be more efficient for the ex vivo expansion of HPCs. These results strongly suggest that MFCs are more suitable for HPCs mass production.

Keywords
CD34+ cells; Cytokine; Expansion; Hematopoietic stem cell; Mesenchymal stem cell; Mixed feeder cells
First Page Preview
Effects of mixed feeder cells on the expansion of CD34+ cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 3, March 2012, Pages 389–394
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering