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Gene cloning and biochemical characterization of a catalase from Gluconobacter oxydans

Paper ID Volume ID Publish Year Pages File Format Full-Text
21283 43215 2011 6 PDF Available
Title
Gene cloning and biochemical characterization of a catalase from Gluconobacter oxydans
Abstract

Gluconobacter oxydans has a large number of membrane-bound dehydrogenases linked to the respiratory chain that catalyze incomplete oxidation of a wide range of organic compounds by oxidative fermentation. Because the respiratory chain is a primary site of reactive oxygen species (ROS) production, the bacterium is expected to have a high capacity to detoxify nascent ROS. In the present study, a gene that encodes a catalase of G. oxydans, which might act as a potential scavenger of H2O2, was cloned, and the expression product (termed rGoxCat) was characterized biochemically. rGoxCat is a heme b-containing tetrameric protein (molecular mass, 320 kDa) consisting of identical subunits. The recombinant enzyme displayed a strong catalase activity with a kcat of 6.28 × 104 s− 1 and a Km for H2O2 of 61 mM; however, rGoxCat exhibited no peroxidase activity. These results, along with the phylogenetic position of the enzyme, provide conclusive evidence that rGoxCat is a monofunctional, large-subunit catalase. The enzyme was most stable in the pH range of 4–9, and greater than 60% of the original activity was retained after treatment at pH 3.0 and 40°C for 1 h. Moreover, the enzyme exhibited excellent thermostability for a catalase from a mesophilic organism, retaining full activity after incubation for 30 min at 70°C. The observed catalytic properties of rGoxCat, as well as its stability in a slightly acidic environment, are consistent with its role in the elimination of nascent H2O2 in a bacterium that produces a large amount of organic acid via oxidative fermentation.

Keywords
Reactive oxygen species; Gluconobacter oxydans; Catalase; Peroxidase; Monofunctional; Hydrogen peroxide
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Gene cloning and biochemical characterization of a catalase from Gluconobacter oxydans
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 111, Issue 5, May 2011, Pages 522–527
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us