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2D motility tracking of Pseudomonas putida KT2440 in growth phases using video microscopy

Paper ID Volume ID Publish Year Pages File Format Full-Text
21299 43215 2011 7 PDF Available
Title
2D motility tracking of Pseudomonas putida KT2440 in growth phases using video microscopy
Abstract

Pseudomonas putida KT2440 is a gram negative motile soil bacterium important in bioremediation and biotechnology. Thus, it is important to understand its motility characteristics as individuals and in populations. Population characteristics were determined using a modified Gompertz model. Video microscopy and imaging software were utilized to analyze two dimensional (2D) bacteria movement tracks to quantify individual bacteria behavior. It was determined that inoculum density increased the lag time as seeding densities decreased, and that the maximum specific growth rate decreased as seeding densities increased. Average bacterial velocity remained relatively similar throughout the exponential growth phase (~ 20.9 μm/s), while maximum velocities peak early in the exponential growth phase at a velocity of 51.2 μm/s. P. putida KT2440 also favors smaller turn angles indicating that they often continue in the same direction after a change in flagella rotation throughout the exponential growth phase.

Keywords
Pseudomonas putida KT2440; Bacterial motility; Cell growth; Video microscopy
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2D motility tracking of Pseudomonas putida KT2440 in growth phases using video microscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 111, Issue 5, May 2011, Pages 605–611
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us