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Determination of the ribosome-binding sequence and spacer length between binding site and initiation codon for efficient protein expression in Bifidobacterium longum 105-A

Paper ID Volume ID Publish Year Pages File Format Full-Text
21303 43216 2012 3 PDF Available
Title
Determination of the ribosome-binding sequence and spacer length between binding site and initiation codon for efficient protein expression in Bifidobacterium longum 105-A
Abstract

The frequency of the ribosome-binding site (RBS) and spacer length between binding site and initiation codon were analyzed using informatics, and compared the activities of various synthetic RBSs and spacers in Bifidobacterium longum 105-A. As the result, AAGGAG and a 5 nt spacer length produced the most efficient protein expression.

Keywords
RBS; Bifidobacterium; Shine-Dalgarno sequence; Translation
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Determination of the ribosome-binding sequence and spacer length between binding site and initiation codon for efficient protein expression in Bifidobacterium longum 105-A
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 4, April 2012, Pages 442–444
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us