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Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506

Paper ID Volume ID Publish Year Pages File Format Full-Text
21304 43216 2012 6 PDF Available
Title
Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506
Abstract

Phosphite dehydrogenase (PtxD), which catalyzes the nearly irreversible oxidation of phosphite to phosphate with the concomitant reduction of NAD+ to NADH, has great potential for NADH regeneration in industrial biocatalysts. Here, we isolated a soil bacterium, Ralstonia sp. strain 4506, that grew at 45°C on a minimal medium containing phosphite as the sole source of phosphorus. A recombinant PtxD of Ralstonia sp. (PtxDR4506) appeared in the soluble fraction in Escherichia coli. The purified PtxDR4506 showed 6.7-fold greater catalytic efficiency (Vmax/Km) than the first characterized PtxD of Pseudomonas stutzeri (PtxDPS). Moreover, the purified PtxDR4506 showed maximum activity at 50°C, and its half-life of thermal inactivation at 45°C was 80.5 h, which is approximately 3,450-fold greater than that of PtxDPS. Therefore, we concluded that PtxDR4506, which shows high catalytic efficiency, solubility, and thermostability, would be useful for NADH regeneration applications.

Keywords
Phosphite; Phosphite dehydrogenase; NADH regeneration; Biocatalyst, Ralstonia
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Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 4, April 2012, Pages 445–450
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us