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Comprehensive evaluation of leukocyte lineage derived from human hematopoietic cells in humanized mice

Paper ID Volume ID Publish Year Pages File Format Full-Text
21319 43216 2012 7 PDF Available
Title
Comprehensive evaluation of leukocyte lineage derived from human hematopoietic cells in humanized mice
Abstract

Recently, humanized animals whereby a part of the animal is biologically engineered using human genes or cells have been utilized to overcome interspecific differences. Herein, we analyzed the detail of the differentiation states of various human leukocyte subpopulations in humanized mouse and evaluated comprehensively the similarity of the leukocyte lineage between humanized mice and humans. Humanized mice were established by transplanting human CD34+ cord blood cells into irradiated severely immunodeficient NOD/Shi-scid/IL2Rγnull (NOG) mice, and the phenotypes of human cells contained in bone marrow, thymus, spleen and peripheral blood from the mice were analyzed at monthly intervals until 4 months after cell transplantation. The analysis revealed that transplanted human hematopoietic stem cells via the caudal vein homed and engrafted themselves successfully at the mouse bone marrow. Subsequently, the differentiated leukocytes migrated to the various tissues. Almost all of the leukocytes within the thymus were human cells. Furthermore, analysis of the differentiation states of human leukocytes in various tissues and organs indicated that it is highly likely that the human-like leukocyte lineage can be developed in mice.

Keywords
Humanized mice; Leukocyte lineage; Hematopoietic stem cell; Cell transplantation; NOG mice
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Comprehensive evaluation of leukocyte lineage derived from human hematopoietic cells in humanized mice
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 4, April 2012, Pages 529–535
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us