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Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen

Paper ID Volume ID Publish Year Pages File Format Full-Text
21320 43216 2012 6 PDF Available
Title
Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen
Abstract

Lactic acid bacteria (LAB) represent an attractive delivery vehicle for oral allergy vaccine because of their safety as a food microorganism as well as their potent adjuvant activity triggering anti-allergic immune response. Here, we report the generation of recombinant LAB expressing a major Japanese cedar pollen allergen Cry j 1 (Cry j 1-LAB), and their prophylactic effect in vivo. To facilitate heterologous expression, the codon usage in the Cry j 1 gene was optimized for the host LAB strain Lactobacillus plantarum by the recursive PCR-based exhaustive site-directed mutagenesis. Use of the codon-optimized Cry j 1 cDNA and a lactate dehydrogenase gene fusion system led to a successful production of recombinant Cry j 1 in L. plantarum NCL21. We also found that oral vaccination with the Cry j 1-LAB suppressed allergen-specific IgE response and nasal symptoms in a murine model of cedar pollinosis.

Keywords
Allergen; Codon optimization; IgE; Japanese cedar pollen; Lactobacillus
First Page Preview
Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 4, April 2012, Pages 536–541
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering