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Efficient purification of native recombinant proteins using proteases immobilized on cellulose

Paper ID Volume ID Publish Year Pages File Format Full-Text
21321 43216 2012 3 PDF Available
Title
Efficient purification of native recombinant proteins using proteases immobilized on cellulose
Abstract

Cellulose binding domain (CBD) fusion protease was employed to digest the CBD fusion protein. After cleavage, the tag free target proteins can be separated from the CBD tag and CBD fusion protease which still adsorbed to the cellulose by centrifugation. The green fluorescent protein was efficiently purified using this method.

Keywords
Cellulose binding domain; Protein purification; Immobilized protease; 3C protease; Cellulose
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Efficient purification of native recombinant proteins using proteases immobilized on cellulose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 4, April 2012, Pages 542–544
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us