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Deletion analysis of the promoter of Aspergillus oryzae gene encoding heat shock protein 30

Paper ID Volume ID Publish Year Pages File Format Full-Text
21323 43217 2009 7 PDF Available
Title
Deletion analysis of the promoter of Aspergillus oryzae gene encoding heat shock protein 30
Abstract

In order to find a promoter that could be influenced by temperature shift, we explored and isolated an Aspergillus oryzae gene expressed at high temperatures (37–42 °C) by the cDNA subtraction method. Of the 96 cDNA clones isolated from the subtraction library, one cDNA clone showed 73% identity with Aspergillus nidulans heat shock protein 30 (hsp30). Based on this, we designated the isolated gene hsp30 of A. oryzae. A. oryzae hsp30 was weakly expressed at 30 °C, but strongly at 40 °C. We showed that the promoter of this hsp30 induced heterologous gene expression at high temperatures using β-glucuronidase (GUS) gene as a reporter. Regarding elucidation of the region essential for heat shock response, we showed that the minimum length of the promoter region that was essential for heat shock response was located between − 388 and − 272 (+ 1 indicated the first position of the translation initiation codon) of the hsp30 promoter. This promoter region harbors several putative transcription factor binding sites, including heat shock elements (HSEs), a CCAAT box, and a TATA box. Furthermore, site-directed mutagenesis of this promoter revealed that HSE1 (aTTCgtcGAAacgcccaGAAa) and HSE2 (cGAAagTTCtcGACg), located between − 342 and − 272 of the hsp30 promoter, were its cis-acting elements for heat shock response.

Keywords
Heat shock protein; Promoter; Heat shock element; β-glucuronidase; Aspergillus oryzae
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Deletion analysis of the promoter of Aspergillus oryzae gene encoding heat shock protein 30
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 4, April 2009, Pages 345–351
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us