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Gene cloning and characterization of a xylanase from a newly isolated Bacillus subtilis strain R5

Paper ID Volume ID Publish Year Pages File Format Full-Text
21326 43217 2009 6 PDF Available
Title
Gene cloning and characterization of a xylanase from a newly isolated Bacillus subtilis strain R5
Abstract

A novel mesophilic strain, R5, was isolated from Osaka, Japan. The growth temperature of the strain ranged from 10 to 40 °C with optimal growth at 30 °C. Cells of strain R5 were highly motile small rods. The full-length 16S rRNA sequence was 99% homologous to that of Bacillus subtilis strain 168. The optimum pH and NaCl concentration for growth of the strain were 7.0 and 3%, respectively. Based on the biochemical characteristics and 16S rRNA sequences R5 was identified as a strain of B. subtilis. The strain R5 produced protease, cellulase, amylase, lipase/esterase, xylanase and a biosurfactant extracellularly. The gene encoding xylanase was cloned and expressed in Escherichia coli. The gene encoded a protein consisting of 213 amino acids with a relative molecular mass of 23 kDa. The gene product was purified and examined for enzymatic characteristics. The recombinant enzyme exhibited highest activity at temperatures ranging from 40 to 50 °C and at pH 6.0. The enzyme activity was enhanced in the presence of metal cations. The Vmax and Km values of the recombinant enzyme towards xylan from beech-wood were 5550 nkat/mg and 4.5 mg/mL, respectively.

Keywords
Bacillus subtilis R5; 16S rRNA; Extracellular enzymes; Xylanase; Cloning; Kinetic parameters
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Gene cloning and characterization of a xylanase from a newly isolated Bacillus subtilis strain R5
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 4, April 2009, Pages 360–365
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us