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Cost effective DNA sequencing and template preparation from bacterial colonies and plasmids

Paper ID Volume ID Publish Year Pages File Format Full-Text
21344 43217 2009 3 PDF Available
Title
Cost effective DNA sequencing and template preparation from bacterial colonies and plasmids
Abstract

Cost-effective DNA sequencing and template preparation were evaluated for high-throughput screening of bacterial colonies. The rolling-circle amplification to generate template for DNA sequencing was carried out using 10-fold smaller amount of phi29 DNA polymerase and excluding two other enzymes (yeast pyrophosphatase and calf intestine alkaline phosphatase) used previously. Then, a 1/40 volume of the fluorescent terminator mix recommended by the manufacturer gave a usable sequencing result although a 1/26.7 volume of the mix was used to compare the protocol more accurately with manufacturer's protocol.

Keywords
Cost-effective; Whole-plasmid amplification; DNA sequencing; Sanger methods; Phi29 DNA polymerase
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Cost effective DNA sequencing and template preparation from bacterial colonies and plasmids
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 4, April 2009, Pages 471–473
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us