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Improved yields of cyclic nigerosylnigerose from starch by pretreatment with a thermostable branching enzyme

Paper ID Volume ID Publish Year Pages File Format Full-Text
21358 43218 2010 7 PDF Available
Title
Improved yields of cyclic nigerosylnigerose from starch by pretreatment with a thermostable branching enzyme
Abstract

Cyclic nigerosylnigerose (CNN) is produced enzymatically from starch by the combined action of 6-α-glucosyltransferase and 3-α-isomaltosyltransferase. In our previous study, α-1,6-branching chains found in the structure of amylopectin and glycogen were shown to be favorable for CNN formation by the two enzymes. Therefore, we examined whether the introduction of α-1,6-branch points into starch using the action of branching enzyme (BE) could improve the yield of CNN from starch. Thermostable BE from Geobacillus stearothermophilus TC-91 was prepared as a purified recombinant protein. Pretreatment of amylose with BE considerably increased the CNN yield from 5% to 38%. When BE acted on tapioca starch, the CNN yield was elevated from 47% to 60%. Conversely, BE treatment of waxy corn starch containing very little amylose resulted in a negligible increase in CNN yield. In addition, BE exerted a beneficial effect when starch with a lower degree of hydrolysis was used as a substrate. The present results indicate that the addition of α-1,6-glucosidic linkages to starch using BE is an effective strategy to improve the yield of CNN from starch.

Keywords
CNN, cyclic nigerosylnigerose, cyclo{→6}-α-d-Glcp-(1→3)-α-d-Glcp-(1→6)-α-d-Glcp-(1→3)-α-d-Glcp-(1→); BE, branching enzyme; CGTase, cyclomaltodextrin glucanotransferase; 6GT, 6-α-glucosyltransferase; IMT, 3-α-isomaltosyltransferase; DS, dry solidCyclic nig
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Improved yields of cyclic nigerosylnigerose from starch by pretreatment with a thermostable branching enzyme
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 4, April 2010, Pages 381–387
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us