Evaluation of photo-induced cellular damage using photoautotrophic cultures of puk-bung hairy roots as a sensing tool
In photoautotrophic cultures of pak-bung hairy roots, strong light irradiation (22 W/m2) increased the content of reactive oxygen species (ROS) in the cells, resulting in chlorophyll (Chl) degradation and DNA injury. The Chl degradation rate, RD, can be used as a parameter to measure the cellular damage caused by photo-induced stress. The presence of the antioxidants ascorbic acid, chlorogenic acid or catechin reduced the RD, while lowering the content of ROS and moderating the DNA injury.
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 4, April 2010, Pages 392–394