fulltext.study @t Gmail

Hydrodynamic conditions induce changes in secretion level and glycosylation patterns of Von Willebrand factor (vWF) in endothelial cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
21362 43218 2010 7 PDF Available
Title
Hydrodynamic conditions induce changes in secretion level and glycosylation patterns of Von Willebrand factor (vWF) in endothelial cells
Abstract

The effect of low-speed agitation on the level of secretion and glycosylation patterns of the Von Willebrand factor in endothelial cells cultured in 6-cm Petri dishes located in a rotating platform was studied. In this simple system, complex and non-intuitive velocity fields arise. As revealed by simple calculations of tangential velocities and CFD analysis, flow behavior strongly varies as a function of radial distance and rotational velocity. We found significant differences in both secretion level and glycosylation extent and completeness, as a result of relatively small changes in rotational speed. Results obtained support the hypothesis that differences in hydrodynamic conditions may induce important changes in the secretion and assembly of glycoproteins in mammalian cell cultures.

Keywords
Endothelial cells; Mammalian cells; Mixing; Glycosylation patterns; Von Willebrand factor; Shear stress; Computational fluid dynamics
First Page Preview
Hydrodynamic conditions induce changes in secretion level and glycosylation patterns of Von Willebrand factor (vWF) in endothelial cells
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 4, April 2010, Pages 400–406
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us