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Ionic liquid/water interfacial localization of a green fluorescent protein fused to a tryptophan-rich peptide

Paper ID Volume ID Publish Year Pages File Format Full-Text
21370 43219 2012 6 PDF Available
Title
Ionic liquid/water interfacial localization of a green fluorescent protein fused to a tryptophan-rich peptide
Abstract

We report that several tryptophan-rich peptides exhibit an affinity for a hydrophobic ionic liquid (IL) (1-ethyl-3-methylimidazolium bis-trifluoromethanesulfonyl imide), and that green fluorescent protein (GFP) fused to a peptides, “SSSWWSWWWW” (SW1) or “SWWWWSWWWW” (SW2), containing serine (S) and tryptophan (W) at the C terminus localized at the IL/water interface. While GFPs without W-rich peptide distributed only in water phase, SW1- and SW2-GFPs were accumulated at the interface. The localization of SW1-GFP showed biphasic behavior, and most distinctive localization was observed at 7.1 μM. The localization of SW2-GFP presumably occurred at largely lower concentration (≤ 0.5 μM) than that of SW1-GFP, which difference was due to the higher hydrophobicity of SW2 peptide.

Keywords
1-ethyl-3-methylimidazolium bis-trifluoromethanesulfonyl imide, [Emim][Tf2N]; IL, ionic liquid; FITC, fluorescein isothiocianate; CLSM, confocal laser scanning microscope; GFP, green fluorescent proteinInterface; Ionic liquid; Peptide; Dynamic equilibrium
First Page Preview
Ionic liquid/water interfacial localization of a green fluorescent protein fused to a tryptophan-rich peptide
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 113, Issue 2, February 2012, Pages 160–165
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering