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Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4

Paper ID Volume ID Publish Year Pages File Format Full-Text
21396 43220 2010 4 PDF Available
Title
Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4
Abstract

This is the first report that Lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. Growth of the E4 Lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53 mM ammonium ion concentration) in media containing glucose rather than other tested carbon sources. The optimum glucose concentration was 0.30% at an initial medium pH of 7.0 and incubation temperature of 30 °C. From time-course experiments, when the glucose in the culture was exhausted, ammonia began to be accumulated, and maximum ammonia accumulation (∼ 1.60 mM) was reached after 8 days of incubation. Ammonia accumulation by this strain required molybdenum, manganese, and iron.

Keywords
Ammonia accumulation; Lysobacter; Nitrogen-fixing bacteria
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Ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, Lysobacter sp. E4
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 4, October 2010, Pages 415–418
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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