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In vitro osteogenic differentiation of HOS cells on two types of collagen gels

Paper ID Volume ID Publish Year Pages File Format Full-Text
21405 43220 2010 8 PDF Available
Title
In vitro osteogenic differentiation of HOS cells on two types of collagen gels
Abstract

HOS cell is a model strain of human osteoblasts derived from human osteosarcoma. We cultured the HOS cells on both the conventional collagen gel (neutral gel), and the gamma-crosslinked collagen gel without collagen fibrils (acidic gel). The shape of HOS cells on the neutral gel was similar to that on the culture dish. However, HOS cells on acidic gel had an elongated shape and attached each other to form a mesh-like pattern. The cells attached to the surface of both gels but scarcely penetrated their depths. We measured the biochemical markers for osteogenic differentiation in the HOS cells cultured on both the neutral gel and the acidic gel. The expressions of alkaline phosphatase and osteocalcin were detected in the HOS cells on both types of collagen gel. Deposition of the calcium also occurred on both gels although it was higher in the neutral gel than the acidic one. These results indicate the importance of collagen for the differentiation of HOS cells, but it is not dependent on the molecular structure (fibril formation) of collagen.

Keywords
HOS cell; Osteoblast; Osteogenesis; Calcium; Collagen; Collagen fibril
First Page Preview
In vitro osteogenic differentiation of HOS cells on two types of collagen gels
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 4, October 2010, Pages 471–478
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering