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Production and characterization of alcohol oxidase from Penicillium purpurescens AIU 063

Paper ID Volume ID Publish Year Pages File Format Full-Text
21414 43221 2009 5 PDF Available
Title
Production and characterization of alcohol oxidase from Penicillium purpurescens AIU 063
Abstract

A filamentous fungus belonging to Penicillium purpurescens was isolated as a strain grown well in methanol medium. The strain produced an alcohol oxidase (AOD), which oxidizes short-chain primary alcohols and ethylene glycol, by incubation with a medium containing not only methanol, but also ethanol, ethylene glycol, glycerol or glucose. This AOD belonged to the same group as AOD from methylotrophic yeast, but some properties were different from AODs from methylotrophic yeast and other fungi. Thus, this enzyme consisted of four identical subunits of 66 kDa, while AODs from methylotrophic yeasts were of eight subunits. The enzyme activity was enhanced to two-fold by incubation at pH 6.0 and 40 °C for 60 min, whereas the Km values for ethanol and ethylene glycol did not change.

Keywords
Alcohol oxidase; Penicillium purpurescens; Ethylene glycol; Glycolaldehyde; Primary alcohol; Methylotrophic yeast
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Production and characterization of alcohol oxidase from Penicillium purpurescens AIU 063
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 2, February 2009, Pages 108–112
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us