Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression
We developed a novel in vitro antibody (Ab) generation system using a hypermutating chicken B cell line (DT40-SW). We suppressed the expression of the Pax5 transcription factor by targeted disruption of the gene to increase Ab production in isolated clones and produce the desired Abs. This single genetic manipulation resulted in a significant enhancement of Ab production without significantly affecting maximum cell density.
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 2, February 2009, Pages 206–209