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Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression

Paper ID Volume ID Publish Year Pages File Format Full-Text
21430 43221 2009 4 PDF Available
Title
Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression
Abstract

We developed a novel in vitro antibody (Ab) generation system using a hypermutating chicken B cell line (DT40-SW). We suppressed the expression of the Pax5 transcription factor by targeted disruption of the gene to increase Ab production in isolated clones and produce the desired Abs. This single genetic manipulation resulted in a significant enhancement of Ab production without significantly affecting maximum cell density.

Keywords
Antibody; Chicken B cell line; Plasma cell; Pax5; Gene knockout
First Page Preview
Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 107, Issue 2, February 2009, Pages 206–209
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering