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Activity and stability enhancement of α-amylase treated with sub- and supercritical carbon dioxide

Paper ID Volume ID Publish Year Pages File Format Full-Text
21436 43222 2011 6 PDF Available
Title
Activity and stability enhancement of α-amylase treated with sub- and supercritical carbon dioxide
Abstract

Various physical, chemical and genetic approaches have been applied in order to enhance enzyme stability and activity. In this study, the aim was to investigate the capability of sub- and supercritical carbon dioxide to alter the stability and activity of α-amylase as an alternative technique. The effects of operational parameters such as pressure (50–300 bar), temperature (28–80°C), CO2 flow (2–10 gmin− 1) and time (60–180 min) were evaluated in regard to the activity and stability of fungal based α-amylase from Aspergillus oryzea. The activity of untreated enzyme was determined as 17,726 μmol/ml/min. While both sub- and supercritical conditions enhanced the activity, the increase in flow rate had an adverse effect and the activity was decreased by 28.9% at a flow rate of 10 gmin− 1 under supercritical conditions. Nuclear magnetic resonance (NMR) spectra of untreated enzyme and treated samples exhibiting the lowest and the highest activities were almost identical except for the chemical shifts observed at the lowest activity sample from 4.0 to 4.4 ppm which were assigned to protons of hydrogen-bonded groups. Optimum conditions were determined as 240 bar, 41°C, 4 gmin− 1 CO2 flow and 150 min of process duration yielding 67.7% (29,728 μmol/ml/min) higher activity than the untreated enzyme providing fundamental basis for enzymatic applications.

Keywords
Supercritical fluid processsing; Optimization; Amylase; Enzyme activity; Nuclear magnetic resonance (NMR); Scanning electron microscopy (SEM)
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Activity and stability enhancement of α-amylase treated with sub- and supercritical carbon dioxide
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 5, November 2011, Pages 435–440
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us