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The 184th residue of β-glucosidase Bgl1B plays an important role in glucose tolerance

Paper ID Volume ID Publish Year Pages File Format Full-Text
21438 43222 2011 4 PDF Available
Title
The 184th residue of β-glucosidase Bgl1B plays an important role in glucose tolerance
Abstract

Bgl1B (ACY09072) is a new GH1 family β-glucosidase derived from a marine microbial metagenomic library. In our previous study, the activity of recombinant Bgl1B was competitively inhibited by glucose with an IC50 value of 30 mM. Based on amino acids sequence alignment with other GH1 β-glucosidases, including BglB from Paenibacillus polymyxa whose structure has been solved, the 184th and 409th residues of Bgl1B were suggested to be key residues relating to glucose tolerance or other properties. The putative role of the two residues of Bgl1B was investigated by site-directed mutagenesis by replacing His184 and Leu409 with Phe and Glu, respectively. Biochemical characterization data indicated that the mutations had little influence on the enzyme properties. However, in comparison with wild-type Bgl1B, the mutant H184F exhibited lower stability at all tested temperatures and pHs, while L409E exhibited higher stability. Particularly, when using 4-Nitrophenyl-β-D-glucopyranoside (pNPG) as substrate, the mutant H184F exhibited much better glucose tolerance, with a Ki of 76.9 mM, than L409E and wild-type Bgl1B (17.2 mM and 14.9 mM, respectively). These results indicated that the 184th residue might play an important role in the glucose tolerance of Bgl1B.

Keywords
β-Glucosidase; Competitive inhibition; GH1; Glucose tolerance; Mutation
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The 184th residue of β-glucosidase Bgl1B plays an important role in glucose tolerance
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 5, November 2011, Pages 447–450
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us