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In situ gellable sugar beet pectin via enzyme-catalyzed coupling reaction of feruloyl groups for biomedical applications

Paper ID Volume ID Publish Year Pages File Format Full-Text
21446 43222 2011 4 PDF Available
Title
In situ gellable sugar beet pectin via enzyme-catalyzed coupling reaction of feruloyl groups for biomedical applications
Abstract

In situ gellable hydrogels are more attractive in many biomedical and biopharmaceutical applications than pre-formed hydrogels because they can be implanted simply by injection and allow homogeneous incorporation of bioactive materials. In this study, the potential suitability of in situ gellable sugar beet pectin (SBP) for biomedical and biopharmaceutical applications was investigated. SBP aqueous solution gelled within 1 min after addition of appropriate amounts of horseradish peroxidase (HRP) and H2O2via HRP-catalyzed oxidative coupling reaction of feruloyl groups on SBP molecules. The resultant gels gradually degraded under simulated physiological condition. L929 fibroblast cells encapsulated in the gels were scarcely damaged during the gelation process. A subcutaneously injected mixture of SBP, HRP and H2O2 solutions successfully gelled, and the gel did not induce necrosis in the surrounding tissue 1 week after implantation. These results demonstrate that the in situ gellable SBP gels are useful for biomedical and biopharmaceutical applications.

Keywords
In situ gelation; Sugar beet pectin; Hydrogel; Peroxidase; Oxidative coupling polymerization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 5, November 2011, Pages 491–494
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us