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Mitochondrial development of the in vitro hepatic organogenesis model with simultaneous cardiac mesoderm differentiation from murine induced pluripotent stem cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
21447 43222 2011 6 PDF Available
Title
Mitochondrial development of the in vitro hepatic organogenesis model with simultaneous cardiac mesoderm differentiation from murine induced pluripotent stem cells
Abstract

Induced pluripotent stem (iPS) cells, resembling embryonic stem (ES) cells in many phenomena, including differentiation potential, colony morphology, and the expression of specific representative markers, were generated from differentiated somatic cells by exogenous expression of several transcriptional factors. In recent, the mitochondria of iPS cells were also reported to be rejuvenated to that of ES cells, however it is not known if the mitochondria have same potential for differentiation as ES cells. We have established the murine ES cell-derived in vitro hepatic organogenesis model, consisting of not only hepatocytes but also endothelial networks together with cardiac mesoderm differentiation, previously. By measuring oxygen concentration and pH in the culture medium, respectively corresponding to the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR), we compared the metabolic patterns and bio-energetic profiles of both iPS and ES cells during the hepatic differentiation. The bio-energetic profiles of the in vitro hepatic organogenesis from iPS cells accorded with each differentiation steps, from proliferation stage as the initiation, spontaneously beating cardiac differentiation in the next, and finally liver tissue-formation, as well as that from ES cells. Both iPS and ES cells were differentiated into liver-like tissue with similar mitochondrial development.

Keywords
iPS cell; Mitochondria; Liver; Organogenesis; Cardiac mesoderm; Endothelial network; Oxygen consumption rate; Extracellular acidification rate
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Mitochondrial development of the in vitro hepatic organogenesis model with simultaneous cardiac mesoderm differentiation from murine induced pluripotent stem cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 5, November 2011, Pages 495–500
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us