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Geometric microenvironment directs cell morphology on topographically patterned hydrogel substrates

Paper ID Volume ID Publish Year Pages File Format Full-Text
2145 101 2010 10 PDF Available
Title
Geometric microenvironment directs cell morphology on topographically patterned hydrogel substrates
Abstract

Cell behavior is influenced by numerous factors in the physical environment, and a deep understanding of these interactions can lead to the design of better scaffolds for tissue engineering. In vitro substrates can be used to evaluate a wide range of factors, such as topography, and identify which show promise for further evaluating in vivo. Polyacrylamide hydrogels featuring a combinatorial, micropatterned array of posts with varied shape, width, and spacing were produced using a one-step technique. Substrates were covalently modified with collagen and seeded with D1 ORL UVA mesenchymal stem cells. Patterning was shown to direct several quantitative measures of cell morphology. Cell bodies tended to be located in gaps 15 μm and wider, but on top of posts when gaps were 5 μm and smaller. Cells on substrates with square posts and narrow gaps tended to elongate in the direction of gaps. Finally, smaller gaps on all substrates were also shown to influence the placement of cell extensions. The parameters identified may be incorporated into substrates to direct specific aspects of cell morphology.

Keywords
Micropatterned substrate; Cell morphology; Hydrogel; Topography
First Page Preview
Geometric microenvironment directs cell morphology on topographically patterned hydrogel substrates
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 6, Issue 9, September 2010, Pages 3514–3523
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering