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Development of a novel aptamer-based sensing system using atomic force microscopy

Paper ID Volume ID Publish Year Pages File Format Full-Text
21450 43222 2011 4 PDF Available
Title
Development of a novel aptamer-based sensing system using atomic force microscopy
Abstract

Atomic force microscopy (AFM) can dynamically detect the adhesion or affinity force between a sample surface and a cantilever. This feature is useful as a detection method using aptamers — single-strand DNA that recognizes its target with very high affinity. The present study proposes a novel DNA aptamer-based sensing system using AFM. In this study, thrombin was chosen as the target molecule, and a DNA aptamer-based AFM sensing system based on competition was developed. The affinity force between the gold chip and the cantilever decreased as the concentration of thrombin increased. Moreover, a low detection limit of 0.2 nM was achieved. Therefore, the AFM sensing system used would be appropriate for the measurement of various chemical compounds.

Keywords
DNA aptamer; Atomic force microscopy; Molecular interaction; Force curve measurement; Competitive assay
First Page Preview
Development of a novel aptamer-based sensing system using atomic force microscopy
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 5, November 2011, Pages 511–514
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering