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Strategy for the extraction of yeast DNA from artisan agave must for quantitative PCR analysis

Paper ID Volume ID Publish Year Pages File Format Full-Text
21452 43222 2011 4 PDF Available
Title
Strategy for the extraction of yeast DNA from artisan agave must for quantitative PCR analysis
Abstract

An efficient method for the direct extraction of yeast genomic DNA from agave must was developed. The optimized protocol, which was based on silica-adsorption of DNA on microcolumns, included an enzymatic cell wall degradation step followed by prolonged lysis with hot detergent. The resulting extracts were suitable templates for subsequent qPCR assays that quantified mixed yeast populations in artisan Mexican mezcal fermentations.

Keywords
Yeast DNA extraction; Optimization strategy; Agave must; mezcal fermentation; qPCR
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Strategy for the extraction of yeast DNA from artisan agave must for quantitative PCR analysis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 112, Issue 5, November 2011, Pages 518–521
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us