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Thermostable Kunitz trypsin inhibitor with cytokine inducing, antitumor and HIV-1 reverse transcriptase inhibitory activities from Korean large black soybeans

Paper ID Volume ID Publish Year Pages File Format Full-Text
21454 43223 2010 7 PDF Available
Title
Thermostable Kunitz trypsin inhibitor with cytokine inducing, antitumor and HIV-1 reverse transcriptase inhibitory activities from Korean large black soybeans
Abstract

A large number of trypsin inhibitors belonging to various types have been purified from different kinds of legumes. In this study, by using liquid chromatography, a Kunitz type trypsin inhibitor (KBTI) with a molecular weight of 20107.645 Da was purified from Korean large black soybeans. KBTI reduced the proteolytic activities of trypsin and α-chymotrypsin with the activity of ∼ 8520 BAEE units/mg and ∼ 24 BTEE units/mg, respectively. It showed high thermal stability (0–100 °C) as well as stability over a large range of pH values (pH 3–11). Furthermore, KBTI inhibited HIV-1 reverse transcriptase activity with an IC50 value of 0.71 μM and induced the release of pro-inflammatory cytokines such as TNF-α, IL-1β, IL-2 and interferon-γ at the mRNA level. KBTI exerted weak antiproliferative activity toward CNE-2 and HNE-2 nasopharyngeal cancer cells, MCF-7 breast cancer cells, and Hep G2 hepatoma cells. KBTI was destitute of mitogenic, ribonuclease and antifungal activities.

Keywords
Korean large black soybean; Glycine max; Seeds; Purification; Trypsin inhibitor; Biological properties
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Thermostable Kunitz trypsin inhibitor with cytokine inducing, antitumor and HIV-1 reverse transcriptase inhibitory activities from Korean large black soybeans
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 3, March 2010, Pages 211–217
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us