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An extracellular endo-1,4-β-xylanase from Aspergillus japonicus: Purification, properties, and characterization of the encoding gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
21457 43223 2010 3 PDF Available
Title
An extracellular endo-1,4-β-xylanase from Aspergillus japonicus: Purification, properties, and characterization of the encoding gene
Abstract

SummaryAn extracellular endo-1,4-β-xylanase with specific activity of 566 U/mg was purified from the culture filtrate of a filamentous fungus, Aspergillus japonicus strain MU-2, grown on oat spelt xylan. The purified enzyme showed a single band on SDS–PAGE with an apparent Mr of 25.1 kDa. Xylanase activity was optimal at pH 5.0 and 60 °C. The xylanase gene (xynA) encoded a 42 residue prepropeptide and a 191 residue mature protein. The XynA protein showed the highest sequence identity of 69% to Aspergillus niger XynB (DQ174549), which belongs to the glycoside hydrolase family 11.

Keywords
Aspergillus japonicus; Endo-1,4-β-xylanase; Extracellular enzyme; Glycoside hydrolase family 11; Xylan
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An extracellular endo-1,4-β-xylanase from Aspergillus japonicus: Purification, properties, and characterization of the encoding gene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 3, March 2010, Pages 227–229
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us