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Changes in the quality of antibodies produced by Chinese hamster ovary cells during the death phase of cell culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
21467 43223 2010 7 PDF Available
Title
Changes in the quality of antibodies produced by Chinese hamster ovary cells during the death phase of cell culture
Abstract

Although overproduction of recombinant proteins by mammalian cells is well established, little attention has been paid to analysis of the quality of the products. We focused on the quality of antibodies produced during the death phase of a recombinant Chinese hamster ovary (CHO) cell line. The quality of the monoclonal antibody against HM1.24 antigen and the post-translational characteristics of the subunits during CHO cell culture in a 160-L bioreactor were investigated. The culture supernatant of a stable cell line was collected and purified by affinity chromatography and then analyzed. There were no significant changes in gel-permeation chromatography variables, carbohydrate structure, or antibody-dependent cellular cytotoxicity activity during the death phase of cell culture. However, ion-exchange chromatography analysis revealed that antibody heterogeneity changed, as indicated by a decrease in cell viability. The results presented here provide useful information that will help in determining the time to end each batch culture.

Keywords
Recombinant CHO cell; Monoclonal antibody; HM1.24 antigen
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Changes in the quality of antibodies produced by Chinese hamster ovary cells during the death phase of cell culture
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 109, Issue 3, March 2010, Pages 281–287
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us