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Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
21491 43224 2011 11 PDF Available
Title
Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells
Abstract

Potassium ion (K+) plays vital roles in many aspects of cellular homeostasis including competing with sodium ion (Na+) during potassium starvation and salt stress. Therefore, one way to engineer plant cells with improved salt tolerance is to enhance K+ uptake activity of the cells, while keeping Na+ out during salt stress. Here, in search for Na+-insensitive K+ transporter for this purpose, bacterial expression system was used to characterize two K+ transporters, OsHAK2 and OsHAK5, isolated from rice (Oryza sativa cv. Nipponbare). The two OsHAK transporters are members of a KT/HAK/KUP transporter family, which is one of the major K+ transporter families in bacteria, fungi and plants. When expressed in an Escherichia coli K+ transport mutant strain LB2003, both OsHAK transporters rescued the growth defect in K+-limiting conditions by significantly increasing the K+ content of the cells. Under the condition with a large amount of extracellular Na+, we found that OsHAK5 functions as a Na+-insensitive K+ transporter, while OsHAK2 is sensitive to extracellular Na+ and exhibits higher Na+ over K+ transport activities. Moreover, constitutive expression of OsHAK5 in cultured-tobacco BY2 (Nicotiana tabacum cv. Bright Yellow 2) cells enhanced the accumulation of K+ but not Na+ in the cells during salt stress and conferred increased salt tolerance to the cells. Transient expression experiment indicated that OsHAK5 is localized to the plant plasma membrane. These results suggest that the plasma-membrane localized Na+ insensitive K+ transporters, similar to OsHAK5 identified here, could be used as a tool to enhance salt tolerance in plant cells.

Keywords
K+ transport; KT/HAK/KUP transporter; Na+ transport; Salt tolerance; Genetic engineering; Rice; Tobacco; Yeast; Escherichia coli
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Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 111, Issue 3, March 2011, Pages 346–356
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us