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Trans-acting RNAs as molecular probes for monitoring time-dependent structural change of an RNA complex adapting two structures

Paper ID Volume ID Publish Year Pages File Format Full-Text
21494 43224 2011 7 PDF Available
Title
Trans-acting RNAs as molecular probes for monitoring time-dependent structural change of an RNA complex adapting two structures
Abstract

ABSTRACTAs dynamic structural changes are pivotal for the functions of some classes of RNA molecule, it is important to develop methods to monitor structural changes in RNA in a time-dependent manner without chemical modification. Based on previous reports that trans-acting RNAs can be used as probes for analysis and control of 3D structures of target RNAs, we applied this method to monitor time-dependent structural changes in RNA. We designed and performed a proof-of-principle study using a simple model RNA complex that adopts two different structures as a target. The time-dependent structural changes in the target RNA were successfully monitored using two trans-acting RNAs, which stably form a ternary complex with the bimolecular target RNA and act as a catalyst to join two RNA fragments of the target complex, respectively.

Keywords
RNA structure; Structural change; RNA–RNA interaction; Ribozyme; Riboswitch
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 111, Issue 3, March 2011, Pages 370–376
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us