fulltext.study @t Gmail

Biosurfactant-producing yeast isolated from Calyptogena soyoae (deep-sea cold-seep clam) in the deep sea

Paper ID Volume ID Publish Year Pages File Format Full-Text
21502 43225 2010 7 PDF Available
Title
Biosurfactant-producing yeast isolated from Calyptogena soyoae (deep-sea cold-seep clam) in the deep sea
Abstract

We describe a detailed structure determination of biosurfactant produced by Pseudozyma hubeiensis SY62, which was newly isolated from Calyptogena soyoae (deep-sea cold-seep clam, Shirouri-gai) at 1156 m in Sagami bay. P. hubeiensis SY62 was taxonomically slightly different from the P. hubeiensis type strain, which produces biosurfactants. Glycolipid production by the strain was also slightly different from those of previously reported strains. BS productivity was estimated to be around 30 g/l from the weight of the crude extract. At least five different spots of glycolipid biosurfactants (BSs) were detected by TLC. Results of nuclear magnetic resonance spectroscopies indicated the major product, namely MEL-C (4-O-[4′-O-acetyl-2′,3′-di-O-alka(e)noil-β-d-mannopyranosyl]-d-erythritol), as a promising BS. By further structural determination, the major fatty acids of MEL-C were estimated to be saturated C6, C10, and C12 acids, which were shorter than those of previously reported MEL-C. Furthermore, 1H-NMR spectra implied the presence of C2 acids as acyl groups. According to surface tension determination, the novel MEL-C showed larger critical micelle concentration (1.1 × 10− 5 M) than conventional MEL-C which bound C10 and C12 acids (9.1 × 10− 6 M). From these results, shorter fatty acids would confer hydrophilicity onto the novel MEL-C.

Keywords
Extremobiosphere; Biosurfactant; Mannosylerythritol lipid; Vegetable oil; Pseudozyma; Calyptogena soyoae
First Page Preview
Biosurfactant-producing yeast isolated from Calyptogena soyoae (deep-sea cold-seep clam) in the deep sea
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 2, August 2010, Pages 169–175
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us