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Potential of Psidium guajava Supplemented Fish Diets in Controlling Aeromonas hydrophila Infection in Tilapia (Oreochromis niloticus)

Paper ID Volume ID Publish Year Pages File Format Full-Text
21517 43226 2008 6 PDF Available
Title
Potential of Psidium guajava Supplemented Fish Diets in Controlling Aeromonas hydrophila Infection in Tilapia (Oreochromis niloticus)
Abstract

Fourteen herbs were extracted in water, 95% ethanol, and ether, and tested in vitro for antimicrobial activities against Aeromonas hydrophila, a fish pathogen causing motile Aeromonas septicemia. Using swab paper disc assays and minimal inhibitory concentration (MIC) determinations, we noted that the ethanol extract of Psidium guajava leaf exhibited the strongest antimicrobial activity. The extract allowed growth-inhibited A. hydrophila cells to regrow in fresh BHI broth indicating a bacteriostatic mode of action. In a pathogenicity test, the median lethal dose (LD50) of A. hydrophila for tilapia (Oreochromis niloticus) by intraperitoneal injection was 3.44×106 CFU/ml. In vivo experiments showed that fish diets containing either dry leaf powder of P. guajava or dried ethanol extract of P. guajava leaf reduced mortality of A. hydrophila infected tilapia with no detected adverse effect on the fish. This study suggests that P. guajava leaf has the potential to control fish diseases caused by A. hydrophila.

Keywords
Psidium guajava; Aeromonas hydrophila; motile Aeromonas septicemia; antimicrobial activity; tilapia
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Potential of Psidium guajava Supplemented Fish Diets in Controlling Aeromonas hydrophila Infection in Tilapia (Oreochromis niloticus)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 5, November 2008, Pages 419–424
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us