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Isolation of Industrial Strains of Aspergillus oryzae Lacking Ferrichrysin by Disruption of the dffA Gene

Paper ID Volume ID Publish Year Pages File Format Full-Text
21526 43226 2008 5 PDF Available
Title
Isolation of Industrial Strains of Aspergillus oryzae Lacking Ferrichrysin by Disruption of the dffA Gene
Abstract

Based on studies using laboratory strains, the efficiency of gene disruption in Aspergillus oryzae, commonly known as koji mold, is low; thus, gene disruption has rarely been applied to the breeding of koji mold. To evaluate the efficiency of gene disruption in industrial strains of A. oryzae, we produced ferrichrysin biosynthesis gene (dffA) disruptants using three different industrial strains as hosts. PCR analysis of 438 pyrithiamine-resistant transformants showed dffA gene disruption efficiency of 42.9%–64.1%, which is much higher than previously reported. Analysis of the physiological characteristics of the disruptants indicated that dffA gene disruption results in hypersensitivity to hydrogen peroxide. To investigate the industrial characteristics of dffA gene disruptants, two strains were used to make rice koji and their properties were compared to those of the host strains. No differences were found between the dffA gene disruptants and the host strains, except that the disruptants did not produce ferrichrysin. Thus, this gene disruption technique is much more effective than conventional mutagenesis for A. oryzae breeding.

Keywords
Aspergillus oryzae; ferrichrysin; dffA gene; breeding of koji mold; ferrichrysin biosynthesis
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Isolation of Industrial Strains of Aspergillus oryzae Lacking Ferrichrysin by Disruption of the dffA Gene
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Bioscience and Bioengineering - Volume 106, Issue 5, November 2008, Pages 488–492
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us